Figure 3

Histopathology assessment of AGD infection (H&E staining). (A) Sample from negative control fish at 2 dpi showing non-infected gills with normal lamellar structure. Scale bar = 300 μm. (B) Sample from infected fish at 2 dpi showing infection signs such as mild focal hyperplasia and fusion of lamellar epithelium (*). Scale bar = 200 μm. (C) Sample from GS 2 fish (field sampling) showing moderate multifocal hyperplasia and fusion of lamellar epithelium (*) with associated presence of amoeboid organisms (arrow heads). Scale bar = 200 μm. (D) Histology sample taken 4 days after freshwater treatment with less prominent hyperplastic foci. Note regression of inter-lamellar cell mass (arrows). Scale bar = 200 μm.