Figure 5 | Scientific Reports

Figure 5

From: Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

Figure 5

Nucleotide sequences around the target site in the potato GBSSI gene from the generated representative mutants. The corresponding wild-type nucleotide sequence (WT) is shown in the upper portion of the figure. The nucleotide sequences of the gRNAs (#1, #2, and #3) are indicated above. The region corresponding to the HindIII site is shaded and indicated by bold letters. Numbers with the prefix # indicate the individual transformants. When multiple DNA sequences were detected, they were aligned. Gaps indicate a nucleotide deletion in the mutants. Nucleotide insertion and replacement is shown as nucleotides on the appended line. Large numbers of nucleotide deletions and insertions are noted on the lines.

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