Figure 3 | Scientific Reports

Figure 3

From: The Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli ΔbioH-complementing esterase EstN1

Figure 3

Auxotrophic insertion mutants of E. coli Rosetta-gami 2 (DE3) bioA, bioB, bioD and bioF were complemented with the corresponding bioA/bioB/bioD/bioF-genes from E. coli and N. gargensis and an empty vector pET21a served as control. The clones were assayed for growth on M9 medium without biotin and under supplementation of 4 nM biotin. All complementation assays were carried out in triplicate in at least three independent experiments.

Back to article page