Table 2 Identification of significantly different potential endogenous metabolites in the sera of ratsa.

From: Renoprotective effect of Zhenwu decoction against renal fibrosis by regulation of oxidative damage and energy metabolism disorder

No.

Identified potential biomarker

Related pathway

Concentration (Intensity/1000)b

Fc

Sig.c

p valued

CON

MOD

ELM

ZWD

CON VS MOD

CON VS ZWD

MOD VS ZWD

1

16(R)-HETE

Arachidonic acid metabolism

15.07 ± 0.99

14.99 ± 0.70

12.89 ± 1.68

12.29 ± 1.73

11.22

2.42E-5

9.01E-1

3.3E-3

3.76E-3

2

LysoPC(18:1)

Glycerophospholipid metabolism

25.03 ± 0.58

31.09 ± 1.27

28.31 ± 0.72

24.35 ± 0.56

140.03

6.65E-20

3.67E-8

8.22E-2

1.25E-8

3

LysoPC(16:0)

Glycerophospholipid metabolism

65.06 ± 1.13

86.32 ± 1.29

82.65 ± 3.99

66.27 ± 1.08

240.93

7.52E-24

3.33E-16

1.33E-1

4.32E-21

4

LysoPC(18:0)

Glycerophospholipid metabolism

9.97 ± 0.15

11.34 ± 0.17

10.37 ± 0.19

8.66 ± 0.10

565.73

2.51E-30

5.77E-15

8.71E-9

2.88E-15

5

LysoPE(18:0)

Glycerophospholipid metabolism

3.52 ± 0.082

2.85 ± 0.046

3.76 ± 0.11

3.59 ± 0.11

189.56

4.19E-22

8.58E-12

6.19E-1

1.72E-9

6

Palmitic acid

Fatty Acid Biosynthesis

7.70 ± 0.40

10.68 ± 0.44

12.10 ± 0.73

10.64 ± 1.04

69.00

5.35E-15

3.62E-11

1.80E-5

1.00

7

Linoleic acid

Linoleic acid metabolism

16.89 ± 0.87

24.01 ± 1.14

27.34 ± 1.65

22.46 ± 1.06

128.75

2.66E-19

1.08E-10

1.65E-9

3.22E-2

8

Stearic acid

Fatty Acid Biosynthesis

1.25 ± 0.09

6.08 ± 0.12

6.58 ± 0.24

5.99 ± 0.28

254.18

2.83E-24

6.65E-16

1.35E-8

5.44E-1

9

EPA

Fatty Acid Biosynthesis

1.91 ± 0.07

2.54 ± 0.25

3.96 ± 0.67

3.72 ± 0.32

61.29

3.21E-14

7.23E-5

5.35E-8

2.96E-7

10

DPA

Fatty Acid Biosynthesis

1.93 ± 0.06

2.48 ± 0.35

2.90 ± 0.45

2.56 ± 0.51

11.004

2.84E-5

4.19E-3

2.00E-2

9.99E-1

11

Adrenic acid

Fatty Acid Biosynthesis

1.08 ± 0.06

1.29 ± 0.09

1.16 ± 0.07

1.42 ± 0.18

17.24

4.17E-7

6.77E-5

1.22E-3

3.38E-1

12

PC(34:2)

Glycerophospholipid metabolism

3.74 ± 0.70

6.67 ± 0.46

6.29 ± 0.41

4.68 ± 0.37

75.50

1.34E-15

4.76E-8

1.17E-2

3.48E-8

13

9(S)-HODE

Linoleic acid metabolism

0.53 ± 0.04

0.78 ± 0.02

0.75 ± 0.08

0.85 ± 0.10

36.89

4.47E-11

2.53E-10

5.75E-1

4.06E-4

14

15S-HETrE

γ-Linolenic acid metabolism

0.28 ± 0.02

0.33 ± 0.02

0.22 ± 0.05

0.27 ± 0.05

21.058

4.74E-8

2.17E-4

9.09E-1

5.46E-3

15

Arachidonic acid

Arachidonic acid metabolism

33.09 ± 0.83

46.87 ± 3.10

16.98 ± 2.14

41.74 ± 1.73

95.27

3.48E-17

3.95E-7

1.45E-8

2.96E-3

  1. aMetabolites were confirmed by tR and m/z with authentic chemicals. bThe concentration of potential biomarkers in sera of rats are presented as the mean ± standard deviation. cThe value of F and Sig. was obtained from a one-way ANOVA. The F value is the ratio of the variance between the groups and the variance in the group. F value greater than 1 and Sig. less than 0.05 means the differences between groups were statistically significant. dp values were calculated from the Dunnett’s t-test in multivariate statistical analysis. 16(R)-HETE, 16R-Hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid; EPA, Eicosapentaenoic acid; DPA, Docosapentaenoic acid; 9(S)-HODE, 9S-Hydroxy-10E,12Z-octadecadienoic acid; 15S-HETrE, 15(S)-Hydroxyeicosatrienoic acid. CON, control group; MOD, model group; ELM, enalapril maleate group; ZWD: ZWD group.
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