Figure 3
From: sox9b is required in cardiomyocytes for cardiac morphogenesis and function
sox9b function is necessary in cardiomyocytes for proper cardiac function. (a–f) Videos of control (Tg(myl7:Gal4VP16;UAS:tRFP)) zebrafish and zebrafish with cardiomyocyte-specific loss of sox9b function (Tg(myl7:Gal4VP16;UAS:dnsox9b-2A-tRFP) were collected and cardiac function was analyzed at 36, 48, and 72 hpf. End-diastolic (EDV) and end-systolic (ESV) volumes were approximated by applying “The Method of Discs” or “Simpson’s Method” to still images of ventricles at peak diastole and systole. EDV and ESV were used to calculate stroke volume (SV; SV = EDV − ESV) and ejection fraction (EF; EF = (EDV − ESV)/EDV × 100). Heart rate was also measured and, along with stroke volume, used to calculate cardiac output (CO; CO = SV x HR). Error bars represent standard error of the mean. Asterisks indicate significant differences between groups as determined by Student’s t-test. (p < 0.05), n = 7 per group. Light bars indicate control group and dark bars indicate the experimental group.
