Figure 2

Morroniside treatment stimulated human ORSC proliferation and migration and increased cells in S and S/G1 phases in vitro. (a) Morroniside increased proliferation of ORSCs in a dose-dependent manner, as shown by MTS assays. (b,c) Distributions of ORSCs in G1, S, and G2 phases in the presence or absence of morroniside were detected by flow cytometry. (d) Fold changes in the fractions of cells in S and S/G1 phases with or without morroniside treatment. (e,f) Transwell assays of ORSCs with or without morroniside treatment. (g,h) Wound scratch assays of ORSCs cultured with or without morroniside treatment. a.u., arbitrary units. MMC, mitomycin C. Bars represent the mean ± SD. *P < 0.05, **P < 0.01.