Figure 6

PFEE-C and PFEE-W induce ER stress in H22 cells. H22 cells were treated with PFEE-C and PFEE-W. After 24 h, proteins were isolated and the levels of ER stress-related proteins were detected by Western blot. Cropped blots are shown and full-length blots are included in the Supplementary Information. Grayscale scanning data were obtained by Image J. The ratios of HSP70/β-actin, P-JNK/β-actin and cleaved-cas-12 /cas-12 were shown in lower panels. Data are from 3 independent experiments and analyzed by ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001 compared to untreated group.