Figure 2 | Scientific Reports

Figure 2

From: Vps34-mediated macropinocytosis in Tuberous Sclerosis Complex 2-deficient cells supports tumorigenesis

Figure 2

Macropinocytosis is further increased by autophagy inhibition in Tsc2-deficient cells. (A) Immunoblots confirmed Atg5 knockout in Tsc2+/+ and Tsc2−/− MEFs. The blot was cropped to highlight the relevant bands. The full-length blot is presented in Supplementary Fig. 3. (B) Macropinocytosis was increased in Tsc2−/− sgAtg5 MEFs compared to Tsc2−/− sgCtl MEFs. Median fluorescent intensity of FITC-Dextran was quantified by flow cytometry. Data represented as mean +/− standard deviation of three biological replicates. Statistical significance was assessed using two-way and one-way ANOVAs with Bonferroni correction with ****p < 0.0001.

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