Figure 6

Effects of NNFE on MAP kinase signalling in melan-a cells. (A) Melan-a cells were co-treated with NNFE and selective inhibitors of ERK (U0126), JNK (SP600125), or p38 (SB239063). MITF and tyrosinase levels were determined by western blot analysis, and (B) melanin content was also determined. Measurements were made in triplicate, and results are expressed as the means ± SDs. #p < 0.05, versus the non-treated controls; **p < 0.01, versus the IBMX-treated controls, Student’s t-test. NNFE, ethyl acetate fraction of N. nouchali flower extract.