Figure 1

The TREX complex associates with the m⁶A methyltransferase complex. (A) WTAP and KIAA1429 co-IP/Western analysis with TREX complex subunits. CONTROL antibody was anti-FLAG throughout the study. Antibodies used for IP are shown above the panels and antibodies used for detection on the right hand side of panels throughout the study. (B) METTL3/METTL14 Co-IP/Western with TREX subunits. (C) Western analysis showing efficient depletion of KIAA1429 by RNAi (upper panel). Combined KIAA1429 and WTAP siRNA treatment resulted in substantial knockdown of both proteins (lower panel). (D) WTAP Co-IP/Western analysis in a KIAA1429 siRNA background with the indicated TREX and m⁶A machinery subunits. (E) METTL3 co-IP/Western analysis with ALYREF. METTL3 antibody was used for the IP. All Co-IP analysis presented in the paper were carried out in the presence of RNase A. Where separate panels are shown for same the protein in Western blots, these are all taken from the same blot at the same exposure. Full size blots are displayed in Supplementary Information.