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Figure 1

From: Cellular Depletion of BRD8 Causes p53-Dependent Apoptosis and Induces a DNA Damage Response in Non-Stressed Cells

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BRD8 knockdown induces cell cycle arrest and cell death. BRD8 depleted in HCT116 cells with two siRNAs targeting BRD8 (siBRD8-35 and siBRD8-36) or control siRNA (Ctrl) for 48 h. (a) Expression levels of BRD8 in HCT116 p53+/+ cells before and after knockdown using two independent siRNAs. (b) Immunoblot showing BRD8 expression in BRD8 knockdown cells. Numbers represent densitometric analysis normalized to actin. (c) Crystal violet staining viability assay of HCT116 p53+/+ cells at 24, 48, 72 and 96 h post transfection with siRNAs targeting BRD8 (siBRD8-35 and siBRD8-36) or control siRNA (Ctrl). Three independent experiments were performed in technical duplicates; the mean ± SD is shown. (d) Cell cycle distributions of HCT116 p53+/+ following BRD8 knockdown for 72 h and analyzed by PI staining FACS. The mean of 3 independent experiments ± SD is shown. An ANOVA test followed up with a Dunnett analysis was used to compare each mean to its relative control. *P ≤ 0.05; **P ≤ 0.01; ns = non-significant compared to relative control.

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