Figure 4 | Scientific Reports

Figure 4

From: Cellular Depletion of BRD8 Causes p53-Dependent Apoptosis and Induces a DNA Damage Response in Non-Stressed Cells

Figure 4The alternative text for this image may have been generated using AI.

Knockdown of BRD8 activates p53 in HCT116 p53+/+ cells. (a–d) BRD8 was depleted in HCT116 cells with two siRNAs targeting BRD8 (siBRD8-35 and siBRD8-36) or control siRNA (Ctrl) for 48 h. Total RNA was extracted and RT-qPCR assays were performed. mRNA expression levels of p21, p53, MDM2 and p27 were monitored. The mean ± SD from three independent experiments are shown. Total cell extracts of HCT116 p53+/+ (e) and HCT116 p53−/− (f) were subjected to immunoblot assays using the indicated antibodies. The graphics represent densitometric analyses normalized to actin or GAPDH. An ANOVA test followed up with a Dunnett analysis was used to compare each mean to its relative control. *P ≤ 0.05; **P ≤ 0.01; ns = non-significant compared to relative control.

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