Figure 5 | Scientific Reports

Figure 5

From: Cellular Depletion of BRD8 Causes p53-Dependent Apoptosis and Induces a DNA Damage Response in Non-Stressed Cells

Figure 5The alternative text for this image may have been generated using AI.

Lack of p21 does not rescue proliferation arrest and apoptosis in BRD8-depleted cells. (a) Crystal violet staining assay of HCT116 p21−/− cells at 24, 48, 72 and 96 h post transfection with BRD8-targeting (siBRD8-35 and siBRD8-36) or control (Ctrl) siRNAs. Three independent experiments were performed; the mean ± SD is shown. (b) Apoptosis in HCT116 p21−/− cells were quantified by FACS analysis of Annexin V and PI double staining 72 h post transfection. (c) Cell cycle distributions of HCT116 p21−/− following BRD8 knockdown for 72 h and analyzed by PI staining FACS. The mean of three independent experiments ± SD is shown. Two-sample t-Test assuming unequal variances was used for statistical analysis. *P ≤ 0.05; **P ≤ 0.005 compared to control.

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