Figure 7 | Scientific Reports

Figure 7

From: Cellular Depletion of BRD8 Causes p53-Dependent Apoptosis and Induces a DNA Damage Response in Non-Stressed Cells

Figure 7The alternative text for this image may have been generated using AI.

BRD8 knockdown activates CHK2 but abrogates CHK1. Total cell extracts of HCT116 p53+/+ (a and b) and p53−/− (e and f) were subjected to immunoblot assays using indicated antibodies 48 h post-transfection with BRD8-targeting (siBRD8-35 and siBRD8-36) or control (Ctrl) siRNAs. The graphs represent densitometric analyses normalized to GAPDH. (c and d) RT-qPCR assay showing mRNA expression levels of CHK1 and E4F1. The mean ± SD from three independent experiments are shown. The arrows indicate hyper-phosphorylated RPA32 species containing pT21.

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