Figure 1 | Scientific Reports

Figure 1

From: Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells

Figure 1

Rotavirus infection-induced phosphorylation of MLC in polarized epithelial cells is an early event. (a and b) Human RVA DS-1 and bovine RVA NCDV strains (MOI = 10) were inoculated into confluent MDCK monolayers, and cells were harvested at the indicated time points. Cell lysates were subjected to western blot analysis to determine expression levels of phosphorylated MLC (pMLC) and MLC2 using the relevant antibodies. GAPDH was used as a loading control. The intensity of pMLC relative to GAPDH was determined by densitometric analysis and is indicated above each lane. All experiments were performed in triplicate and representative images of different gels from each group are presented.

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