Figure 5 | Scientific Reports

Figure 5

From: Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells

Figure 5

Inhibition of the RhoA/ROCK/MLC pathway restores tight junction resistance in rotavirus-infected polarized epithelial cells. (a and b) MDCK monolayers grown on transwell filters were pretreated with or without RhoA inhibitor (CT04), ROCK inhibitor (Y27632), or MLC inhibitor (blebbistatin) for 1 h at 37 °C and then mock-inoculated or inoculated with RVA strain (a) DS-1 or (b) NCDV (MOI = 10), followed by determination of TER at the indicated time points. Net TER was calculated by subtracting the background (membrane filter without cells) and multiplying the resistance (Ω) by the area (0.33 cm2) of the filter. All experiments were performed in triplicate. Data are presented as the mean ± standard error of the mean from three independent experiments.

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