Figure 6
From: Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis
Microbiota induces a transient increase in colonic epithelial cell IL-18 at day 7 post-colonization. (a) Heat map of significantly altered Log2 transformed inflammation-associated genes in the colonic IEL-enriched compartment as assessed by NanoString at days 1 and 7 post-colonization (D1 and D7, respectively, followed by sample number 1–6). Evaluation of IL-18 protein expression by IHC and representative images (b) post-colonization and (c) in SPF mice. Each data point represents one mouse; the horizontal line and whiskers depict the mean ± SD. Data was collected over 2–5 independently executed experiments. Significance was determined by Kruskal-Wallis test with Dunn’s post-hoc test or an unpaired, two-tailed Student’s t test.
