Figure 8
From: Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis
Barrier adaptations post-colonization increase resistance to DSS-induced injury. SPF mice and mice at days 1 and 7 post-colonization (PC) were given 2% DSS in drinking water for 5 days and sacrificed 2 days thereafter to assess mucosal injury, inflammation, and bacterial translocation. Mice given water were used as controls for each group and pooled together. (a) Microscopic injury score and (b) representative haematoxylin and eosin stained images. (c) Stool consistency score. (d) Colon length. (e) Blood in stool score. (f) Percent weight change. (g) Quantification of anaerobic bacteria in the liver and (h) spleen. DL = detection limit (3.33 log CFU/mg of tissue). Each data point represents one mouse; the horizontal line and whiskers depict the mean ± SD. Data was collected over 2 independently executed experiments. Significance was determined by Kruskal-Wallis test with Dunn’s post-hoc test.
