Table 1 Primer sequences and probes used in this study.
Genes | Forward (5′–3′) | Reverse (5′–3′) | Probe |
|---|---|---|---|
IL-6 | CTGCAAGTGC ATCATCGTTGT | TGTCTATACCAC TTCACAAGTCGGA | CAGAATTGCCATTGC ACAACTCTTTTCTCA |
TNF-α | TGTCTACTGAAC TTCGGGTGAT | AACTGATGA GAGGGAGGCCAT | TCCCCAAAGGGAT GAGAAGTTCCCAA |
TLR4 | CCATGCCATGCC TTGTCTT | GAGCTTCAACCC CTTGAAGATCT | TCAATTTCACACC TGGATAAATCCAGCCA |
NF-κB | TGTCTGCCTCTCTCG TCTTCCT | CACCACTGTCACA GACGCTGT | CGAGGCAGCACATAG ATGAACTCCGG |
18S | ATGAGTCCACTT TAAATCCTTTAACGA | CTTTAATATACGC TATTGGAGCTGGAA | ATCCATTGGAGG GCAAGTCTGGTGC |
CCL2 | GTTGGCTCAGCC AGATGCAG | GTAGCTCTCCAGCC TACTCATTG | CCCACTCACCTGC TGCTACTCATTCACC |
HO-1 | CAGGGTGACAGA AGAGGCTAAGAC | TTGTGTTCCTCTG TCAGCATCAC | TCCTGCTCAACATT GAGCTGTTTGAGGA |
Sirt1 | CAGCATCTTGCCTG ATTTGTAAATAC | CACCGAGGAACTA CCTGATTAAAAA | TCTCCACGAACAGC TTCACAATCAACT |
NLRP3 | AGGACCCACAGTGT AACTTGC | CAGAGGTCAGAGC TGAACAACA | TAAGGCCGGAATTC ACCAACCCCAGCT |
