Figure 1 | Scientific Reports

Figure 1

From: Mechanistic insights into the host-microbe interaction and pathogen exclusion mediated by the Mucus-binding protein of Lactobacillus plantarum

Figure 1

Purification and western blotting of MBP-Mubs5s6 protein. Elution profile of Mubs5s6 purification by (A) anion-exchange chromatography: Lane 1 represents the partial purification of MBP-Mubs5s6 protein; (B) hydrophobic interaction chromatography, separation of MBP protein from MBP-Mubs5s6 fusion protein preparations: Lane 1 at 50 mM, Lane 2 at 0 mM concentration of (NH4)2SO4; (C) Gel Filtration chromatography: Lanes 1 and 2 represent purified MBP-Mubs5s6 protein of size of about 82 kDa; (D) Western blot of the purified protein: Lane 1 indicates Mubs5s6 protein. Lane M indicates marker lane.

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