Figure 5
From: Analysis of the role of GSK3 in the mitotic checkpoint
GSK3 inhibition decreases BubR1 and Bub1 levels at the kinetochores in the presence of spindle toxins. (A,B) HeLa cells exposed to 2 μM Nocodazole with or without SB, were subjected immunofluorescence analysis for BubR1 or Bub1 levels, ACA, a kinetochore marker, and DAPI to stain chromosomes. MG132 was added to inhibit the proteasome. (C) MEFs were exposed to Taxol for 12–14 hours, then immunofluorescence analysis performed to obtain BubR1 kinetochore intensity. ACA was used as a kinetochore marker. ~10 cells per condition were imaged using confocal, using maximum projection images of z-stacks to analyze kinetochores. ~300 kinetochores were analyzed for each condition.
