Figure 3
Increased fibrotic tissue formation and hNIS positive cells in rotator cuff injury tissue from a Col1α1-hNIS transgenic rat. Shoulder tissue was collected 8 days following RC injury for histological analysis from a representative Col1α1-hNIS transgenic rat. (a,e) Masson Trichrome stained images of the humeral head area of the shoulder tissue (20× magnification). HH, humeral head; ST, supraspinatus tendon; FT, Fibrotic tissue outlined with white dotted line. The yellow dotted line designates the supraspinatus tendon (ST) and the red arrow demonstrates the location where the tissue was cut to induce supraspinatus tendon injury in the left shoulder of the Col1α1-hNIS transgenic rat. (b,f) 100× magnification of the ST labeled region in 20x images (a and e). (c,d,g,h) are confocal images (400X magnification) following immunofluorescent staining of the control and injured shoulder tissue paraffin embedded rotator cuff tissue sections with Hoechst stain for nuclei (blue- c and g) or an affinity purified polyclonal anti-hNIS antibody (green- d and h). (i) Quantitative RT-PCR analysis for type 1 alpha 1 collagen and hNIS mRNA showed increase in hNIS mRNA in injured shoulder compared to control shoulder of transgenic rats (n = 5) compared to WT rats (n = 3). P = 0.05 as determined by Student’s t-test analysis.
