Figure 3 | Scientific Reports

Figure 3

From: Reversible Human Immunodeficiency Virus Type-1 Latency in Primary Human Monocyte-Derived Macrophages Induced by Sustained M1 Polarization

Figure 3

Upregulation of HIV-1 restriction factors in M12 MDM. (A) Expression of APOBEC3A (A3A) and APOBEC3G (A3G). Cytokine stimulation induced the expression of A3A and upregulated that of A3G both before infection and 8 days after. The quantification of the original Western blot shown as Fig. 5S was obtained with the use of the ImageJ software (version 1.47 v, WS Rasband ImageJ, NIH; http://rsb.info.nih.gov). (B) Upregulation of TRIM22 and CIITA mRNA expression. Cytokine stimulation induced the expression of both negative regulators of proviral transcription before and 8 days after infection; the levels of mRNA expression of TRIM22, but not those of CIITA, were also increased in CTR MDM 7 days after infection. Bar graphs represent the fold difference as compared to CTR cells at day 0 prior to infection. Statistical analysis by One-way ANOVA; *p < 0.05, **p < 0.01; ***p < 0.001 (n = 4).

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