Figure 4
From: A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
iRFP imaging system enables the observation of the differences in macrophage-rich plaque burden in vivo. The iRFP → LDLR−/− mice were fed with different HCD quantities for 8 weeks to induce different atherosclerosis plaque burdens. The “ND group” was fed the ND for entire experimental period, the “HCD/ND group” was fed the HCD and ND on alternating weeks, and the “HCD group” was fed the HCD for the entire experimental period. (A) In vivo IVIS images, ex vivo aorta IVIS images (scale bar, 5 mm, signal-positive areas are marked by arrows), same aortas stained with ORO (scale bar, 5 mm, ORO-positive areas are marked by arrows), and aortic root sections stained with ORO (scale bar, 100 \(\mu \)m). (B) ORO-positive areas after 8 weeks of induction plotted against the feeding group. The ORO-stained areas showed a positive correlation with the feeding group at the P = 0.01 level. The data are from one representative experiment of at least two independent experiments.
