Figure 2

MICU1 is stabilized by MICU2. HeLa cells were transfected with MICU1-HA and MICU2-FLAG. After 24 hours, cells were treated with 100 μg/ml Cycloheximide and either untreated or treated with 10 μM MG123. Cells were harvested and lysed after the indicated time points and subjected to western blot analysis with the indicated antibodies. (A) Representative western blot showing the half-life, with or without MG123, upon co-expression of both MICU1 and MICU2. Densitometry analysis of MICU1 (B) and MICU2 (C) protein levels, normalized to endogenous MCU protein, in cells co-expressing MICU1 and MICU2. (D) Non-reducing western blot analysis and (E) quantification of the MICU1/MICU2 heterodimers. Each bar represents mean +/− S.E.M of at least three independent experiments; *p < 0.05; **p < 0.01. a.u.: arbitrary units.