Figure 3
From: Parkin-dependent regulation of the MCU complex component MICU1
MICU1 is ubiquitinated. HeLa cells were transfected with MICU1-HA or pcDNA3.1 as control. MG132 treatment for 2 hours was used to increase the amount of ubiquitinated proteins. Ubiquitin (Ub) was immunoprecipitated from whole cell lysate with a specific agarose-Tandem Ubiquitin Binding Entities (TUBE). The precipitated proteins were immunoblotted with α-HA to observe the MICU1-HA-Ub complex. Asterisk indicates non-specific bands.
