Figure 4
From: Parkin-dependent regulation of the MCU complex component MICU1
Parkin-dependent regulation of the MCU complex components. HeLa cells were transfected with the indicated Parkin constructs and either MICU1-HA or MICU2-flag. 24 hours after transfectioncells were treated for 2 hours with 10 μM CCCP (where indicated), medium was changed in control groups. Total proteins were extracted and subjected to western blot analysis with the indicated antibodies. Representative western blot image showing the effect of different Parkin constructs (WT, G430D and ΔUbl) on MICU1 (A) MICU2 (C) and endogenous MCU (E) protein levels. Densitometric analysis of MICU1 (B) MICU2 (D) and endogenous MCU (F) protein levels normalized to endogenous MCU protein, in cells co-expressing MICU1-HA or MICU2-flag along with the indicated Parkin constructs. Each bar represents mean +/− S.E.M of at least three independent experiments; * p < 0.05; *** p < 0.005. a.u.: arbitrary units.
