Figure 6
From: Parkin-dependent regulation of the MCU complex component MICU1
Co-immunoprecipitation analysis of Parkin and the MCU complex components. HeLa cells were transfected with the indicated constructs. Overnight treatment with MG123 was used to prevent Parkin-mediated degradation of MICU1-HA. Parkin was immunoprecipitated from whole cell lysate with anti-Parkin antibody. (A) The precipitated proteins were immunoblotted with αHA to observe the MICU1-Parkin complex. (B) Ubiquitinated proteins were immunoprecipitated (right panel) from the total lysate (left panel) of HeLa cells overexpressing the indicated constructs, subjected to western blot analysis and probed with the indicated antibodies.
