Figure 3
From: Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1
Aβ peptides inhibit migration of hP1-CL cells. Cell migration was measured as cell movement on glass cover slips in polystyrene dishes at 37 °C and 5% CO2. A PDMS barrier to cell growth was created on the cover slip. When cells reached confluency, the barrier was peeled off. Images of the resulting, initially sharp confluent cell boundary were captured at 0.003 Hz over 5 hours. Top panel shows initial (t = 0 min, black dotted lines) and final time points (t = 240 min, red dotted lines) of cell migration. hP1-CL cell migration was blocked by 5 µM D-GsMTx4, as well as 10 pM concentrations of L and D Aβ(1-40). Bottom left panel summarizes the mean cell migration distances as a function of time. Bottom right panel is the mean migration rate of hP1-CL cells in presence and absence of 5 µM D-GsMTx4, and of 10 pM of the L or D forms of Aβ(1-40). Three independent experiments were averaged (SEM).
