Figure 5
From: Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1
D-GsMTx4 and enantiomeric Aβ(1-40) peptides did not alter actin stress of unmodified HEK293T cells expressing the actin force probe. (Panel A) HEK293T cells were monitored for 30 min to establish a baseline. Upon removal of bath Ca2+, addition of scrambled Aβ peptide, or control bath solution exchange (blue bar), the actin stress probe showed no change in FRET. (Panel B) Addition of D-GsMTx4 (5 µM) or the enantiomeric Aβ(1-40) peptides (10 pM) similarly showed no effect on actin stress in these cells. Panel C summarizing the response of control HEK293T cells (15 individual cells from 3 different experiments with SD) shows that actin stress is unaffected by D-GsMTx4 or enantiomeric Aβ peptides in cells lacking PIEZO1 overexpression.
