Figure 6
Apoptosis in MSA iPSC-derived neurons. (A,B) Representative images of immunocytochemistry for apoptotic neurons using a marker for apoptosis, cleaved-Caspase 3. Neurons that were βIII-tubulin/cleaved Caspase 3-double-positive were considered apoptotic neurons. Clvd casp3, cleaved-Caspase 3. (A) The scale bars represent 200 µm. (B) Images of MSA_A line. The scale bars represent 50 µm. (C) Quantitative data of the ratio of cleaved-Caspase 3-positive cells in βIII-tubulin-positive neurons in glucose-free medium (Gal-M) (n = 3 biological replicates per clone; mean ± SE, *p < 0.05, Dunnett’s test). (D) Effect of glucose-free condition on the ratio of cleaved-Caspase 3-positive cells in βIII-tubulin-positive neurons. Glu-M, normal glucose-containing medium (n = 3 biological replicates per clone; mean ± SE, *p < 0.05, Dunnett’s test). (E) Effect of coenzyme Q10 (CoQ10) treatment on the ratio of cleaved-Caspase 3-positive cells in βIII-tubulin-positive neurons in normal condition (n = 3 biological replicates per clone; mean ± SE, *p < 0.05, paired t-test).
