Figure 2 | Scientific Reports

Figure 2

From: PHD3 regulates glucose metabolism by suppressing stress-induced signalling and optimising gluconeogenesis and insulin signalling in hepatocytes

Figure 2

GCN5–CITED2–PKA signalling module mediates cAMP induction of PHD3 gene expression. (A) Effects of shRNA-mediated CITED2 knockdown on mRNA expression of three PHD isoforms in primary mouse hepatocytes with or without exposure to pCPT-cAMP (100 μM, 6 h). (B) Effects of ectopic CITED2 expression on Phd3 gene expression in primary mouse hepatocytes with or without exposure to pCPT-cAMP (100 μM, 6 h). (C,D) Effects of shRNA-mediated GCN5 (C) or PGC-1α (D) knockdown on Phd3 gene expression in primary mouse hepatocytes with or without exposure to pCPT-cAMP (100 μM, 6 h). Data are shown as mean ± SEM (n = 3) and are representative of at least two independent experiments. Differences between groups were evaluated by ANOVA followed by Bonferroni’s post hoc test. **P < 0.01 vs. indicated groups. Adenoviral vectors encoding CITED2 and shRNAs targeting CITED2, GCN5, and PGC-1α were used for experiments.

Back to article page