Figure 3 | Scientific Reports

Figure 3

From: PHD3 regulates glucose metabolism by suppressing stress-induced signalling and optimising gluconeogenesis and insulin signalling in hepatocytes

Figure 3

PHD3 is required for cAMP-dependent induction of gluconeogenesis. (A,B) Effects of shRNA-mediated PHD3 knockdown on gluconeogenic gene expression (A) and glucose production (B) in primary mouse hepatocytes with or without exposure to pCPT-cAMP for 6 and 22 h, respectively. (C,D) Effects of ectopic expression of shRNA-resistant PHD3(WT) or PHD3(ΔPH) on gluconeogenic gene expression (C) and glucose production (D) in primary mouse hepatocytes with or without shRNA-mediated knockdown of PHD3 in the presence of pCPT-cAMP (100 μM, 6 h). (E) Effects of shRNA-mediated PHD3 knockdown on HIF-1α and -2α protein levels in hepatocytes with or without exposure to DMOG (1 mM, 4 h). α-Tubulin served as the loading control for immunoblotting. Complete immunoblots are presented in Supplementary Fig. S7. Quantitative data are shown as mean ± SEM (n = 3) and are representative of at least two independent experiments. Differences between groups were evaluated by ANOVA followed by Bonferroni’s post hoc test. **P < 0.01 vs. indicated groups. Adenoviral vectors encoding PHD3 shRNA, sh-R PHD3(WT), or sh-R PHD3(ΔPH) were used for experiments.

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