Figure 4

Deletion of Hif-1α attenuates the nuclear accumulation of Lipin1 in CDD-treated livers. (a) Representative immunoblots of lipin1 levels in different subcellular fractions of the livers of mice fed a CDD. NC, mice fed normal chow. (b) Protein levels of lipin1 in different subcellular fractions of the livers of mice fed a CDD quantified using densitometry; n = 5–7 mice per group. *P < 0.05. (c) ChIP-qPCR analysis of lipin1 levels at the promoter of PPARα target genes in the livers of mice fed a CDD; n = 7–8 mice per group. *P < 0.05.