Figure 3
Alteration of the transcriptional landscape of TIGKs infected with P. gingivalis in the presence of absence of active PPAD. Significant changes in GS, e.g., biological processes and pathways, induced in TIGKs upon infection with PPAD-competent P. gingivalis were mapped. The fill color of each node represents the direction and significance level for this comparison (orange, enrichment, FDR q < 0.05; red, enrichment, FDR q < 0.01; magenta, enrichment, FDR q < 0.001; green, depletion, FDR q < 0.05; spring green, depletion, FDR q < 0.01; cyan, depletion, FDR q < 0.001). Alterations observed upon comparing TIGKs infected with P. gingivalis PPADC351A (inactive PPAD) and TIGKs infected with P. gingivalis WT are superimposed on this network. The result of the comparison is visualized by the border color of each node (green, depletion, FDR q < 0.05; gray, unchanged). The alterations significantly dependent on PPAD activity were magnified. For each of these pathways, heatmaps of the genes significantly contributing to their enrichment in TIGKs infected with P. gingivalis WT vs. uninfected cells, and significantly contributing to their depletion in TIGKs infected with P. gingivalis PPADC351A vs. TIGKs exposed to P. gingivalis WT, are given. Each field of the heat map is color coded to represent the per-row z-score of the gene expression value of the respective gene and sample.
