Figure 1
slc15a4 is required for imiquimod induced weight loss and epidermal thickening. slc15a4feeble and WT mice were treated with imiquimod (IMQ) and euthanized after 24 hours or 5 days. (A) Significant IMQ-induced weight loss was observed in WT mice as compared to slc15a4feeble mice (n = 7, 5, 17, 15). (B) Attenuated IMQ-induced acanthosis was observed in slc15a4feeble mice at 24 hours and 5 days post daily IMQ treatment compared to WT mice (n = 7, 5, 6, 6, 17, 5). (C) Representative images of H&E stained mouse dorsal skin from WT and slc15a4feeble mice demonstrated attenuated acanthosis in slc15a4feeble mice compared to WT mice on day 5 (n = 7, 5, 17, 15). Images taken at 200 × . Scale is the same in all pictures; scale bar = 100 µm. Data combined from 3 independent experiments. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001 in unpaired two-sided parametric T tests.
