Figure 3
IMQ-dependent recruitment of CD4 + T cells into the dermis requires slc15a4. slc15a4feeble and WT mice were shaved and treated with imiquimod (IMQ) or only shaved and euthanized after 5 days. (A) Quantitative measurements of CD4 T-cell (CD4), myeloid cell (CD11b) and pDC (Siglec H) infiltrate from immunostained dorsal mouse skin of WT and slc15a4feeble mice 5 days post topical treatment with IMQ or control. n = 4, 5, 4, 5 mice for WT, slc15a4feeble, WT + IMQ, slc15a4feeble + IMQ, respectively. (B) Representative immunostained sections of dorsal mouse skin at 6 days post treatment with IMQ. n = 4, 5, 4, 5 images from left to right. Images taken at 200x. Scale is the same in all pictures; scale bar = 100 µm. Data is from 1 of 3 similar experiments with 4–5 mice/group. *p < 0.05, **p < 0.005, in unpaired two-sided parametric T tests.
