Figure 6
Unexpected more potent induction of trademark psoriasiform genes and inflammatory cytokines over time in skin of slc15a4 deficient mice. RT-qPCR analysis of inflammatory genes elevated in 25plex cytokine analyses in IMQ-treated mice 24 hours (A) and 5 days (D) post treatment with IMQ. IL12b and IL23a were added to address Th1 and Th17 skewing, respectively. Cytokines were rank ordered from greatest to least induction in WT. Expression of trademark psoriasiform genes conserved between human and 5 mouse models is shown rank ordered for human psoriasis at 24 hours (B) and 5 days (E) post-treatment with IMQ. Otherwise, all other datasets are rank ordered for WT mouse induction. IFN-associated genes are shown 24 hours (C) and 5 days (F) post-treatment with IMQ. Fold change = IMQ relative quantity (RQ)/control RQ, see Materials and Methods for further details. Data is representative of 3 combined experiments with 5–13 mice/group. n.d. = not detected. *p < 0.05 in unpaired T-test after multiple comparison correction.
