Figure 3
From: A one-step procedure to probe the viscoelastic properties of cells by Atomic Force Microscopy
The effect of morphology on cell viscoelasticity. (a) Immunofluorescence images of PDAC P53R172H cells on patterned and unpatterned surfaces. Scale bar 20 μm. Notice that only single cells spread to the boundaries of the pattern were analysed. (b) The storage modulus (G′(ω)), the loss modulus (G″(ω)) and loss tangent (G″(ω)/G′(ω)) of cells on patterned and non-patterned substrates. All cell measurements were carried out using an ARROW-TL1 cantilever with an attached 4.7 μm silica bead. 50 randomly selected cells were measured in each condition. Data were given as the mean values (central line) and standard deviations (shade area) in (I) & (II). The mean values were used to calculate the loss tangent. The dotted lines in (I) & (II) indicate different zones as described in the text. The black arrow in (III) shows a transition point.
