Figure 4
Analysis of H&E stained images. (a) Illustration of a frozen section, as well as the approximate locations of the inner and outer subregions in the cell culture sample. H&E stained images of the respective subregions of the M4C1 cell culture sample are also shown. Scale bar = 50 µm. (b) and (d) Number and size of SW480 spheroids found in the M4C1 and M4C2 cell culture samples, respectively. (c) and (e) Histograms of the cell counts in the inner and outer subregions of the M4C1 and M4C2 cell culture samples, respectively. *P < 0.05.
