Figure 2
From: Heterocyclic sterol probes for live monitoring of sterol trafficking and lysosomal storage disorders

The intracellular conversion of FP-5. Cells loaded with FP-5 probe were harvested at various time points (0.5–24 h) and cellular extracts were subjected to MS analysis. The original acetylated form of FP-5 was gradually converted to hydroxyl-derivative as demonstrated by percentage of conversion during time.