Figure 1 | Scientific Reports

Figure 1

From: Paraoxonase 1 knockout rats have impaired T cell development at the CD4/CD8 double-negative to double-positive transition stage

Figure 1

Generation and identification of PON1−/− rats using the CRISPR/Cas9 system. (a) Schematic overview of the strategy used to generate PON1−/− rats. (b) RT-PCR analysis of PON1 mRNA levels in the liver, spleen and thymus of 2-month-old PON1−/− and PON1+/+ rats. (c) Western blot analysis of PON1 protein levels in the liver, spleen and thymus of 2-month-old PON1−/− and PON1+/+ rats. (d) Immunohistochemical analysis of PON1 expression in the cortex and medulla of the thymus in 2-month-old PON1−/− and PON1+/+ rats. GAPDH expression was used for normalization. Scale bar = 20 μm.

Back to article page