Figure 1 | Scientific Reports

Figure 1

From: Partial depletion of CD206-positive M2-like macrophages induces proliferation of beige progenitors and enhances browning after cold stimulation

Figure 1

Effect of cold stimulation on BAT and ingWAT in WT mice and DT-treated CD206DTR transgenic (Tg) mice. (A–C) Changes in body weight (g) and adipose tissue weight (BAT and ingWAT) of WT and Tg mice at RT (white) and at a cold temperature (black) as the percentage of body weight (n = 4–6 mice per group). (D,E) mRNA expression of M2-like macrophage marker genes in the ingWAT and BAT of cold-stimulated WT and Tg mice compared with their littermate controls at RT (n = 3–5 mice per group). (F) Representative flow cytometric images of ingWAT from WT and Tg mice (RT vs. cold) (left panel) showing M2-like macrophages. The M2-like macrophages in CD45+ SVF are quantified in the right panel. After debris removal, live cells were gated for CD45+ cells (hematopoietic) and then for F4/80+CD206+ cells (M2-like macrophages) (n = 3–4 mice per group). An isotype control antibody was used as a negative control. The data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, compared with their littermates, as determined using Student’s t-test (AU: arbitrary unit, ns: non-significant).

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