Figure 2

Partial depletion of CD206⁺ M2-like macrophages increases browning of ingWAT. (A) Representative images of paraffin sections of ingWAT from DT-treated WT and Tg mice after cold exposure (96 h) stained with anti-UCP1 antibody (left panel). UCP1⁺ cells were counted in 5 randomly selected microscopic fields (20×) and were expressed as the average cell number per microscopic field/total ingWAT weight (right panel) (n = 4–6 mice per group; scale bar, 100 μm). (B) mRNA expression of Ucp1 and other browning marker genes in the ingWAT of cold stimulated DT-treated WT and Tg mice (n = 4–6 mice per group). (C,D) Glucose concentrations from an intraperitoneal glucose tolerance test (IP-GTT) (n = 7 mice per group) and glucose concentrations from an intraperitoneal insulin tolerance test (IP-ITT) as the percent of the basal glucose level (n = 7–9 mice per group) in DT-treated WT and Tg mice maintained at a cold temperature. The data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, compared with littermates, as determined using Student’s t-test.