Figure 2
From: Tryptophan 32-mediated SOD1 aggregation is attenuated by pyrimidine-like compounds in living cells
W32 mediated seeded SOD1 aggregation in a protein-only system. (A) Representative ThT binding assay of the seeded aggregation of G93A-W32 and G93A-S32 (colours correspond to labels in panel B). (B) Analysis of the lag-phase and (C) elongation rate from multiple ThT binding assays shows that G93A-S32 is resistant to seeding. Data points represent individual wells from 3 separate biological replicates. (D-E) Electron micrographs of aggregates produced from panel ‘A’ showing clear fibrils for G93A (D) and amorphous structures for G93A-S32 (E). Error bars represent SD of the mean for W32 (n = 12), W32 + seed (n = 12), S32 (n = 7), and S32 + seed (n = 10). See Supplementary Table 1 for more details. Significance was determined using a one-way ANOVA with Tukey’s post-test (ns = not significant; **p < 0.01; ***p < 0.001). Scale bar, 2 µm; insets, 0.5 µm.
