Figure 3
From: Tryptophan 32-mediated SOD1 aggregation is attenuated by pyrimidine-like compounds in living cells
S32 substitution decreases the aggregation propensities of SOD1 mutants in living cells. (A) Time-lapse microscopy of G85R-W32-GFP and G85R-S32-GFP expressing HEK293FT cells shows bright puncta for G85R-W32-GFP but not G85R-W32S-GFP. (B) Quantitation of time-lapse microscopy reveals G85R-S32-GFP (orange) aggregates to a lesser extent compared to G85R (blue). Inset is the rate of inclusions detected per hour. (C) Cells transfected with either G85R-W32-GFP or G85R-S32-GFP were treated with saponin 48 h post-transfection and imaged pre- and post-treatment. (D) Analysis of saponin insoluble GFP signal shows a significantly higher amount of aggregated GFP for G85R-W32-GFP following saponin treatment. Error bars represent SEM of at least 3 biological replicates. Significance was determined using students t-test (***p < 0.001). Scale bar, 50 µm.
