Table 4 Primer sequences of the 18 enzymes related to pAp metabolism.
NO | Gene | Primer Sequence (5′->3′) | NO | Gene | Primer Sequence (5′->3′) | ||
|---|---|---|---|---|---|---|---|
1 | AASDHPPT | F | CAGTGCTTGCTGCTGAACCTGAG | 10 | CHST12 | F | TCTTGTTCACTCCACTGCCTCTATC |
R | GCTTCTGATTGTTTCCCATTCTTTG | R | ATTTTACTCCAGCATTCCCTTCC | ||||
2 | NDST2 | F | GTCCTCCGGGTATGAAGCTAAAA | 11 | CHST15 | F | GAGGACTGAAGGGAACGAAAACTG |
R | CCTCACATCAAAGGTCAGCAAAG | R | CCGTAATGGAAAGGTGATGAGATC | ||||
3 | NDST4 | F | CAGGGACTGAAGAGGAAGATGAA | 12 | TPST1 | F | GGCATGTCTGGTGATTAGTTCTGT |
R | ATGACTCGTTGTGGAAGAGGTGG | R | AGGTTTTGTTGGCTTTGAGGTCC | ||||
4 | HS3ST2 | F | CTGGAGTTTATCCGAGTACACCCG | 13 | TPST2 | F | ATGGAGGTAGGCAAGGAGAAGTG |
R | GGAGCCTCTTGAGTGACAAAGTAGC | R | AAGTCGAGGATGAGCTTGAGTGA | ||||
5 | HS3ST4 | F | GCTCATCATCGGGGTCAAGAAAG | 14 | GAL3ST1 | F | TTGTTCGAGTCCTCCTTCCACTA |
R | CATCCAAAGTCTTGGGCATCACA | R | GTCTTGCAGGAACTCGGTCAGC | ||||
6 | HS3ST5 | F | CTGCTTGAAATGCTGAACCTACA | 15 | PAPSS1 | F | AACAGATGCGGAAACATTACCAG |
R | ACCACTCAATGCCCTTACCATAA | R | CACCTCCATCCAGAAGACAATCA | ||||
7 | HS6ST3 | F | TCTCACTGTGCTTGGCTTCATC | 16 | PAPSS2 | F | GAACCAGCAGAAATCCACCAATG |
R | CTGTGGGCTTCACTATTTTCATCTA | R | CTCCTCCAGGGCAAAACTTATCG | ||||
8 | CHST3 | F | GGTTGGCTGTGCTGGTTACTGAT | 17 | IMPAD1 | F | ACAGGTCGCTCTTCAGACTTTTG |
R | TGTGCCTTTATGATGCTTGTCTATG | R | TCTTGACTCTTATCAGGCACATCC | ||||
9 | CHST7 | F | CTTGCTACCAACAACTGCTGTGC | 18 | BPNT1 | F | ACGAGGGACCTGGGTCTACG |
R | ACTTCATCTCAACCAATGCGTCC | R | GGAGGCTACCAACCGCATCA |
