Figure 2
From: mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis
Loss of mTORC1 function is pre-osteoblasts affects spleen size and structure. (A) Spleen weights, normalised to total body weight, of 4- and 12-week old mice (n = 10–15/group). Representative haematoxylin and eosin-stained spleen sections from (B) 4-week old and (C) 12-week old eYFP-Osx:Cre (CRE), eYFP-Rptorob+/− (HET), and eYFP-Rptorob−/− (HOM) mice (4x magnification). The dotted white outlines indicate white pulp. (D) Quantitation of total white pulp area, expressed as a percentage of total spleen area, in 4- and 12-week old mice (n = 10/group). (E) Quantitation of white pulp follicle number, normalised to total spleen area analysed, in 4- and 12-week old mice (n = 10/group). (F) Quantitation of white pulp follicle size, in 4- and 12-week old mice (n = 10/group). Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, one-way ANOVA with Tukey’s post-hoc test.
