Figure 4
From: mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis
CXCL12 and IL-7 expression is decreased in eYFP-Rptorob+/− and eYFP-Rptorob−/− mice. (A,B) qPCR was performed on RNA extracted from eYFP+ osteoblast cells isolated from eYFP-Osx:Cre (CRE), eYFP-Rptorob+/− (HET), and eYFP-Rptorob−/− (HOM) mice. Levels of CXCL12 and IL-7 mRNA expression were measured, and data were normalised to β-actin. (C,D) Circulating levels of CXCL12 and IL-7 were measured in peripheral blood serum samples collected from 4- and 12-week old mice (n = 5–10/group) using commercial ELISA kits. *p < 0.05, ***p < 0.005, ****p < 0.001, one-way ANOVA with Tukey’s post-hoc test.
