Figure 2

Reference-based and de novo transcriptomic analyses. (a) Total number of expressed genes (TPM ≥ 1) per tissue identified in the reference-based transcriptomic approach. (b) Total number of expressed unigenes (TPM ≥ 16) per tissue identified in the de novo transcriptomic approach. (c.1) Number of up-regulated genes among tissues under control (CN) condition identified in the reference-based transcriptomic analysis. (d.1) Number of up-regulated unigenes (yellow) and corresponding gene models (red) among tissues under CN condition identified in the de novo transcriptomic analysis. (c.2.) Number of up-regulated (red) and down-regulated (blue) DDEGs in stressed tissues identified in the reference-based transcriptomic analysis. (d.2) Number of up-regulated DDEUs (yellow) and corresponding gene models (red), and down-regulated DDEUs (turquoise) and corresponding gene models (blue) in stressed tissues identified in the de novo transcriptomic analysis. DEU/G = Differentially expressed unigenes/genes; DDEU/G = Drought-responsive differentially expressed unigenes/genes. Sample names are described in Table 1. aComparisons to obtain the number of up-regulated genes by tissue type under control condition: L-CN = L-CN_vs_S-CN + L-CN_vs_R-CN; S-CN = S-CN_vs_L-CN + S-CN_vs_R-CN; R-CN = R-CN_vs_L-CN + R-CN_vs_S-CN. bComparisons to obtain the number of up- and down-regulated genes under drought stress: L-10 = L-CN_vs_L-10; L-20 = L-CN_vs_L-20; S-10 = S-CN_vs_S-10; S-20 = S-CN_vs_S-20; R-10 = R-CN_vs_R-10; R-20 = R-CN_vs_R-20.